Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse and Rat. Species reactivity (tested):Human.
Aufreinigung
Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Immunogen
A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR
This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.88 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target
MTOR (mTOR)
(Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR