This antibody recognizes Mouse HIF3A (581-592). A BLAST analysis was used to suggest cross reactivity with HIF3A protein from Mouse (100 % homology) and Rat (91 % homology). Reactivity against human HIF3A is not expected as nlz 75 % homology for the immunizing sequence is noted. No reactivity is expected against other forms of HIF proteins.
Kreuzreaktivität (Details)
Species reactivity (expected):Rat. Species reactivity (tested):Mouse.
Aufreinigung
Immunoaffinity chromatography.
Immunogen
Synthetic peptide corresponding to aa 581-592 of mouse Hif3a
ELISA (1/14,000-1/80,000). Westen blot (1/1,500-1/8,000), Expect a band approximately 72 kDa in size correspondingto Hif3 α protein by western blotting in the appropriate cell lysate or extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.2 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use
Lagerung
4 °C
Informationen zur Lagerung
Store vial at 2 - 8 °C prior to reconstitution. Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. . For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below.
Liu, DU, Huang, Luo, Wu, Chen: "[Mortality analyses for rungsten miners.]" in: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, Vol. 26, Issue 1, pp. 24-8, (2008) (PubMed).
One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits(HIF1-α, HIF2-α, or HIF3-α) bound to the aryl hydrocarbon receptor nuclear translocator(ARNT) that is also known as HIF1-β. HIF-α is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-α may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.Synonyms: BHLHE17, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF-3 alpha, Hypoxia-inducible factor 3 alpha, IPAS, Inhibitory PAS domain protein, MOP7, Member of PAS protein 7, PASD7