BMI1 Antikörper (AA 252-264)

Produktnummer ABIN118020

Produktdetails anti-BMI1 Antikörper

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Bindungsspezifität: AA 252-264

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BMI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This affinity purified antibody is directed against human Bmi1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Orangutan, Canine, Chimpanzee, Froq, Bovine, Cat, Xenopus and Chicken.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 252-264 of Human Bmi1 protein.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000-1/30,000Western blot: 1/500-1/3,000. Expect a band approx. 37 kDa in size corresponding to Bmi1 in theappropriate cell lysate or extract. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 % (w/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Andere Bezeichnung: BMI-1 / RNF51 (BMI1 Produkte)

Hintergrund: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gen-ID: 100532731

NCBI Accession: NP_001190991

UniProt: P35226

Pathways: Zellzyklus, Autophagie