ING5 Antikörper (AA 127-140)

Produktnummer ABIN118053

Produktdetails anti-ING5 Antikörper

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Bindungsspezifität: AA 127-140

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ING5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: Protein Sequence: Human p28 ING5 isoform 1, 240 aa, predicted MW 27.8 kDa: 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 rverlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt kpkgkwfcp rcvqekrkkk Protein Sequence: Human p28 ING5 isoform 2, 226 aa, predicted MW 25.9 kDa 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 verlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt tkpkgk

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2 (see below). However, 100 % homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA (1: 4,000 - 1: 16,000) and byWestern blot (1: 500-1: 1,000, Expect a band approximately 28 kDa or 26 kDa in sizecorresponding to variants of ING5 in the appropriate cell lysate or extract). Also suitable for Immunofluorescence microscopy.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.2 mg/mL (UVabs. 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Details zu ING5

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Andere Bezeichnung: ING5 (ING5 Produkte)

Hintergrund: ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING5 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.Synonyms: Inhibitor of growth protein 5, p28ING5

Gen-ID: 84289

NCBI Accession: NP_115705

UniProt: Q8WYH8

Pathways: Chromatin Binding