Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on Lewis rat splenocytes). Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.