MHC Class II RT-1B Antikörper

Produktnummer ABIN118332

Produktdetails anti-MHC Class II RT-1B Antikörper

Target: MHC Class II RT-1B

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II RT-1B Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat thymocyte membrane glycoproteins

Klon: OX-6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry (1:50 - 1:100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on rat splenocytes. Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100): this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu MHC Class II RT-1B

Target: MHC Class II RT-1B

Andere Bezeichnung: MHC Class II RT1B (MHC Class II RT-1B Produkte)

Hintergrund: Synonyms: HLA Class II, MHC Class 2 RT1B