MHC Class II RT-1B Antikörper

Produktnummer ABIN118333

Produktdetails anti-MHC Class II RT-1B Antikörper

Target: MHC Class II RT-1B

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II RT-1B Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Klon: OX-6

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MHC Class II RT-1B

Target: MHC Class II RT-1B

Andere Bezeichnung: MHC Class II RT1B (MHC Class II RT-1B Produkte)

Hintergrund: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H2 region.Synonyms: HLA Class II, MHC Class 2 RT1B