Ubiquitin Antikörper
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- Target Alle Ubiquitin Antikörper anzeigen
- Ubiquitin
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Reaktivität
- Human, Rind (Kuh)
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Ubiquitin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody recognises ubiquitin.
- Aufreinigung
- Affinity chromatography on Protein G
- Immunogen
- Ubiquitin from bovine erythrocytes conjugated to bovine gamma Globulin. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse PAI myeloma cell line.
- Klon
- 1B4-UB
- Isotyp
- IgG1
- Top Product
- Discover our top product Ubiquitin Primärantikörper
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- Applikationshinweise
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ELISA. Western Blot. Flow Cytometry: 1/10 - 1/50, Membrane permeabilisation is required for this application. Immunohistochemistry on frozen sections: 1/500 - 1/1000. Immunohistochemistry on paraffin sections: 1/500 - 1/1000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Human hypophysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Ubiquitin
- Andere Bezeichnung
- Ubiquitin (Ubiquitin Produkte)
- Hintergrund
- Synonyms: Polyubiquitin-B, UBB
- Gen-ID
- 7314
- UniProt
- P0CG47
- Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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