Flow Cytometry (1/50-1/100): Membrane permeabilisation is required. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry. Immunhistology on Frozen Sections (1/1000-1/5000). Immunhistology on Paraffin Sections (1/500-1/1000): use bone marrow as positive control. ELISA. Western blotting (Non reducing conditions): ABIN119048 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
OBrien, Abdulahad, Rutgers, Huitema, OReilly, Coughlan, Harrington, Heeringa, Little, Hickey: "Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies." in: Scientific reports, Vol. 5, pp. 11888, (2015) (PubMed).
Schreiber, Xiao, Jennette, Schneider, Luft, Kettritz: "C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 2, pp. 289-98, (2009) (PubMed).
Myeloperoxidase/MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kD) and two light chains (15 kD), the heavy chains being linked by a disulphide bond. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase auto-antibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.Synonyms: MPO