Dieser CD40 Ligand Antikörper ist konjugiert mit FITC
Applikation
Flow Cytometry (FACS)
Spezifität
This antibody recognizes the CD154 cell surface antigen. Clone MK13A4 has been reported to block binding of CD40 ligand to its receptor CD40. SM1555A is recommended for function studies.
CD40LG
Reaktivität: Human
FACS, ELISA
Wirt: Maus
Monoclonal
8H10F5
unconjugated
Applikationshinweise
Flow Cytometry: neat - 1:20, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Tris buffered saline containing 0.02 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bose, Dasgupta, Tarafder, Bandyopadhyay: "Microwave-processed nanocrystalline hydroxyapatite: simultaneous enhancement of mechanical and biological properties." in: Acta biomaterialia, Vol. 6, Issue 9, pp. 3782-90, (2010) (PubMed).
Casati, Camisaschi, Novellino, Mazzocchi, Triebel, Rivoltini, Parmiani, Castelli: "Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 6, pp. 3782-8, (2008) (PubMed).
CD154 is a 32kD glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5