WB, ELISA, IP, DB, ISt
Wirt: Maus
Monoclonal
HIS-H8
unconjugated
Applikationshinweise
ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.