Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA: 1 μg/mL - 10 μg/mL. Functional Assays. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 without preservatives., None, None
Konservierungsmittel
Without preservative
Handhabung
This product is photosensitive and should be protected from light
TNF alpha is a 17 KDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immuno-regulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2