This antibody recognizes the Calcitonin Receptor. Clone 31/01 recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b Human isoforms.
Aufreinigung
Affinity Chromatography on Protein A.
Immunogen
Synthetic peptide derived from a sequence within the cytoplasmic domain of Human Calcitonin Receptor. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
ELISA: 1/1000 - 1/5000. Immunohistochemistry on paraffin sections: 1/50 - 1/500, we recommend the use of 4 % paraformaldehyde fixation, rather than formalin fixation, This product does not require protein digestion pre-treatment of paraffin embedded sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Recommended Positive Control: Kidney. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Calcitonin receptor is a membrane protein with seven transmembrane domains that is coupled to G protein messenger systems. The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.Synonyms: CALCR, CT-R, CTR