This antibody recognises CD147 (also known as basigin). OX-114 recognises both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains.
Flow Cytometry: (1/25 - 1/50): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes. Immunohistochemistry on frozen sections. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases.Synonyms: 5F7, BSG, Basigin, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor