This antibody recognises the CD178 cell surface antigen. This antibody may be used in combination with clone 14C2 (MCA2409) in ELISA assays. Clone 10F2 is reported to block the activity of Fas ligand. We recommend the use of SM2268LE for such studies.
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. ELISA. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
FASL
(Fas Ligand (TNF Superfamily, Member 6) (FASL))
CD178 is a 40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6