Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
The antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Aufreinigung
Affinity Chromatography on Protein A
Immunogen
Pellet of porcine brain cold stable proteins after depolymerization of microtubules
NEFM
Reaktivität: Human, Ratte, Maus
IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunohistochemistry on paraffin sections. Immunocytochemistry. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
NEF3 antikoerper, NF-M antikoerper, NFM antikoerper, Nef3 antikoerper, Nfm antikoerper, NF160 antikoerper, NF165 antikoerper, nef-m antikoerper, nef3 antikoerper, nefm antikoerper, nf-m antikoerper, zgc:112359 antikoerper, nefm-a antikoerper, nefm-b antikoerper, neurofilament medium antikoerper, neurofilament triplet M protein antikoerper, neurofilament, medium polypeptide antikoerper, neurofilament medium polypeptide antikoerper, neurofilament medium L homeolog antikoerper, neurofilament, medium polypeptide a antikoerper, neurofilament medium S homeolog antikoerper, NEFM antikoerper, Nefm antikoerper, TVAG_582010 antikoerper, TVAG_379630 antikoerper, nefm.L antikoerper, nefma antikoerper, nefm.S antikoerper
Hintergrund
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide