SYK Antikörper (AA 5-360)

Produktnummer ABIN120732

Produktdetails anti-SYK Antikörper

Target: SYK (Spleen tyrosine Kinase (SYK))

Bindungsspezifität: AA 5-360

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SYK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The monoclonal SYK-01 antibody reacts with Protein Tyrosine Kinase p72Syk (Syk, Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FceRI).

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Recombinant fragment (aa 5-360) of human Syk

Klon: SYK-01

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry. Immunoprecipitation. Western blot: Recommended Dilution: 1-2 mg/mL, 60 min, Positive Control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line. Negative Control: HeLa human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-SYK Antikörper (ABIN120732)

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, (2002) (PubMed).

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, (1989) (PubMed).

Details zu SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Andere Bezeichnung: SYK (SYK Produkte)

Hintergrund: Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FceRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK

Gen-ID: 6850

UniProt: P43405

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling