This antibody is specific for the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154.
ELISA (direct ELISA, 0.5 μg/mL). Western blot (0.1 - 0.2 μg). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 0.1 mL distilled water.
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline pH 7.4, containing no preservatives
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store lyophilized at 2 - 8 °C and reconstituted (undiluted) at -20 °C. Avoid repeated freezing and thawing. For long term storage the addition of 0.09 % sodium azide is recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: One year from despatch.Reconstitution: Restore with 0.1 ml distilled water..
CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5