Immunohistochemistry with formalin fixed paraffin embedded tissues: 5-8 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.7, containing 0.01 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-80 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Encephalopsin, a member of the Opsin subfamily of GPCRs, is similar in sequence to vertebrate retinal and pineal opsins and may affect photoreception in the brain. Encephalopsin expression has been documented in various brain tissues, including the preoptic area and paraventricular nucleus of the hypothalamus, cerebral cortex, cerebellar Purkinje neurons, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. ESTs have been isolated from human blood, breast, cervix, colon, germ cell, head/neck, kidney, lung, pancreas, placenta, prostate, skin, testis, and uterus cancer libraries, as well as from normal human blood, brain, eye, heart, kidney, liver, liver/spleen, ovary, placenta, prostate, skin, testis, and B-cell/lung/testis libraries.Synonyms: ECPN, Encephalopsin, OPN3, Panopsin