Cytokeratin 19 Antikörper (Biotin)
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- Target Alle Cytokeratin 19 (KRT19) Antikörper anzeigen
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Cytokeratin 19 Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Spezifität
- The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.
- Kreuzreaktivität (Details)
- Human
- Aufreinigung
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- Immunogen
- MCF-7 human breast adenocarcinoma cell line
- Klon
- A53-B-A2
- Isotyp
- IgG2a
- Top Product
- Discover our top product KRT19 Primärantikörper
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- Applikationshinweise
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Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL. - Kommentare
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze.
Avoid prolonged exposure to light. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).
: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).
: "
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Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).
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- Target
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
- Andere Bezeichnung
- Cytokeratin 19 (KRT19 Produkte)
- Hintergrund
- Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS
- Gen-ID
- 3880
- UniProt
- P08727
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