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CD11b Antikörper

Name: CD11b Antikörper
Brand: antibodies-online
SKU: ABIN125683
Price: 293.28 EUR
Availability: InStock

ITGAM Reaktivität: Human FACS, IP Wirt: Maus Monoclonal MEM-174 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0,3 μg/mL, GAM APC). (CD11b Antikörper)

FACS

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor. (CD11b Antikörper)

Separation of human monocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11b (MEM-174) purified antibody (concentration in sample 0,3 μg/mL, GAM APC). (CD11b Antikörper)

FACS

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN125683

Datenblatt (PDF)

Target Alle CD11b (ITGAM) Antikörper anzeigen

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Reaktivität
279

175

72

34

20

19

17

14

11

10

7

4

4

2

2

1
Human
Wirt
159

115

105

2

1

1
Maus
Passende Sekundärantikörper
Klonalität
286

94

1
Monoklonal
Konjugat
125

39

32

29

22

11

8

8

7

7

6

6

5

5

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Dieser CD11b Antikörper ist unkonjugiert
Applikation
285

85

85

81

63

50

37

22

22

21

14

14

12

6

5

5

5

4

3

2

2

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP)

Spezifität

The antibody MEM-174 recognizes an extracellular epitope of CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Kreuzreaktivität (Details)

Human

Aufreinigung

Purified by protein-A affinity chromatography.

Reinheit

> 95 % (by SDS-PAGE)

Immunogen

Human granulocytes

Klon

MEM-174

Isotyp

IgG2a

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ITGAM Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-174 FITC

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Applikationshinweise

Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes.
Immunoprecipitation: Positive control: granulocytes, CD11b/CD18 transfectants.
Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 mL of sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel

Azide free

Handhabung

Do not freeze.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, (2015) (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung

CD11b (ITGAM Produkte)

Hintergrund

Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Gen-ID

3684

UniProt

P11215

Pathways

Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation