GST-Tag Antikörper
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- Target Alle GST-Tag Produkte
- GST-Tag
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser GST-Tag Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP)
- Spezifität
- The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
- Kreuzreaktivität (Details)
- Recognizes fusion proteins in all species
- Aufreinigung
- Purified by protein-A affinity chromatography.
- Reinheit
- > 95 % (by SDS-PAGE)
- Immunogen
- GST fusion protein
- Klon
- S-tag-05
- Isotyp
- IgG2b
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- Applikationshinweise
- Western blotting: Recommended dilution: 1 μg/mL, positive control: GST fusion protein, reducing conditions.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not freeze.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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- Target
- GST-Tag
- Andere Bezeichnung
- GST tag (GST-Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
- The GST (glutathione-S-transferase) is a 26 kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
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