Nitrotyrosine Antikörper
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- Target Alle Nitrotyrosine Antikörper anzeigen
- Nitrotyrosine
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Nitrotyrosine Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Produktmerkmale
- Synonyms: NO-Tyrosine, Nitro-Tyrosine
- Aufreinigung
- Subsequent Ultrafiltration and Size Exclusion Chromatography
- Immunogen
- KLH modified with Nitrotyrosine
- Klon
- 7A5
- Isotyp
- IgG1
- Top Product
- Discover our top product Nitrotyrosine Primärantikörper
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- Applikationshinweise
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore with 1 mL H2O (15 min, RT)
- Buffer
- 1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C/-80 °C
- Informationen zur Lagerung
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Haltbarkeit
- 12 months
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- Target
- Nitrotyrosine
- Abstract
- Nitrotyrosine Produkte
- Substanzklasse
- Chemical
- Hintergrund
- The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.Synonyms: NO-Tyrosine, Nitro-Tyrosine
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