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Nitrotyrosine Antikörper

WB, EIA Wirt: Maus Monoclonal 7A5 unconjugated
Produktnummer ABIN126845
  • Target Alle Nitrotyrosine Antikörper anzeigen
    Nitrotyrosine
    Reaktivität
    Bitte anfragen
    Wirt
    • 24
    • 21
    • 5
    Maus
    Klonalität
    • 26
    • 24
    Monoklonal
    Konjugat
    • 20
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Nitrotyrosine Antikörper ist unkonjugiert
    Applikation
    • 48
    • 27
    • 25
    • 19
    • 18
    • 14
    • 13
    • 13
    • 10
    • 10
    • 9
    • 8
    • 3
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Produktmerkmale
    Synonyms: NO-Tyrosine, Nitro-Tyrosine
    Aufreinigung
    Subsequent Ultrafiltration and Size Exclusion Chromatography
    Immunogen
    KLH modified with Nitrotyrosine
    Klon
    7A5
    Isotyp
    IgG1
    Top Product
    Discover our top product Nitrotyrosine Primärantikörper
  • Applikationshinweise
    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL H2O (15 min, RT)
    Buffer
    1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C/-80 °C
    Informationen zur Lagerung
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.
    Haltbarkeit
    12 months
  • Target
    Nitrotyrosine
    Abstract
    Nitrotyrosine Produkte
    Substanzklasse
    Chemical
    Hintergrund
    The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.Synonyms: NO-Tyrosine, Nitro-Tyrosine
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