AKT1
Reaktivität: Human
WB, IHC, IF, IP
Wirt: Kaninchen
Monoclonal
unconjugated
Applikationshinweise
Suitable for Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated HepG2 cells (provided with this antibody). ELISA: 0.05 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll
POSITIVE CONTROL CELL LYSATE: HepG2 untreatedFormat:
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1 mL H2O (15 min, RT).
Lagerung
-20 °C
Informationen zur Lagerung
For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution,aliquote and freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °Cup to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles.
The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase