Phosphothreonine Antikörper (phosphorylated)

Produktnummer ABIN126869

Produktdetails anti-Phosphothreonine Antikörper

Target: Phosphothreonine

Bindungsspezifität: phosphorylated

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphothreonine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat and Dog.

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Klon: 14B3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells. Western Blot (Immunoblotting): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: pSer / pThr Molecular Weight MarkerFormulation:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °Cand reconstituted (aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 1 week.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Produkte

Substanzklasse: Amino Acid

Hintergrund: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.