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Phosphotyrosine Antikörper (phosphorylated) (Biotin)

Name: Phosphotyrosine Antikörper (phosphorylated) (Biotin)
SKU: ABIN126872
Price: 843.23 EUR
Availability: InStock

WB, IP, EIA Wirt: Maus Monoclonal 2C8 Biotin

Western Blotting (WB) image for anti-Phosphotyrosine (phosphorylated) antibody (Biotin) (ABIN126872) (Phosphotyrosine Antikörper (phosphorylated) (Biotin))

1 image

Produktnummer ABIN126872

Datenblatt (PDF)

Target Alle Phosphotyrosine Produkte

Phosphotyrosine
Bindungsspezifität
21

2

1

1

1

1

1

1

1
phosphorylated

Reaktivität

Bitte anfragen
Wirt
88

27

1
Maus
Passende Sekundärantikörper
Klonalität
98

18
Monoklonal
Konjugat
37

16

11

8

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Phosphotyrosine Antikörper ist konjugiert mit Biotin
Applikation
80

60

40

38

34

23

22

16

13

9

7

7

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Aufreinigung

Size exclusion chromatography

Bestandteile

incl. positive Control

Immunogen

Synthetic phosphopeptide conjugated to KLH

Klon

2C8

Isotyp

IgG1
anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, FACS, ICC Wirt: Maus Monoclonal P-Tyr-01 unconjugated

anti-Phosphotyrosine antibody
WB, ELISA Wirt: Maus Monoclonal unconjugated

anti-Phosphotyrosine antibody
Reaktivität: Diverse Spezies WB, IHC (p) Wirt: Maus Monoclonal 3F6 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 unconjugated

anti-Phosphotyrosine antibody
WB, IP, IF, IHC, FACS, ISt, StM Wirt: Maus Monoclonal PY20 unconjugated

anti-Phosphotyrosine antibody (HRP)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 HRP

anti-Phosphotyrosine antibody (FITC)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 FITC

anti-Phosphotyrosine antibody (Biotin)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 Biotin

anti-Phosphotyrosine antibody (PE)
WB, IP, IF, IHC, ICC Wirt: Maus Monoclonal G104 PE

Applikationshinweise

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

0.5 mg/mL

Buffer

PBS/0.09 % Na-Azide/PEG and Sucrose

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C/-80 °C

Informationen zur Lagerung

Store the antibody at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit

12 months
Target

Phosphotyrosine

Abstract

Phosphotyrosine Produkte

Substanzklasse

Amino Acid

Hintergrund

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.