Phosphotyrosine Antikörper (phosphorylated)

Produktnummer ABIN126874

Produktdetails anti-Phosphotyrosine Antikörper

Target: Phosphotyrosine

Bindungsspezifität: phosphorylated

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Aufreinigung: Size exclusion chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Klon: 2C8

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Phosphotyrosine MW standardSize: 20 BlotsFormulation: Phosphotyrosine modified standard proteins lyophilized from PBS / 0. 1 %SDS / PEG / Sucrose / Malachitgreen. The following standard proteins were modified with phosphotyrosine: galactosidase (116kD), phosphorylase A (98 kD), BSA (67 kD), ovalbumin (46 kD), carbonic anhydrase (32kD), and soybean trypsin inhibitor (24 kD). Storage: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The phosphptyrosine molecular weight marker is recommended forimmunoblot applications. Use 20 µl of the phosphotyrosine molecular weight marker perlane (mini gel). The individual proteins of the marker are recognized by the following commerciallyavailable clones: 2C8, 1F9, 3B12, 9F1, 9H8, 16F4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Produkte

Substanzklasse: Amino Acid

Hintergrund: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.