NCOA1 Antikörper (pTyr416)

Produktnummer ABIN126893

Produktdetails anti-NCOA1 Antikörper

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Bindungsspezifität: pTyr416

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NCOA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Spezifität: AM00145PU-N specifically interacts with activated src phosphorylated at Tyr 416 and with src family kinases phosphorylated at the corresponding Tyr in the activation loop. The antibody does not crossreact with the non-phosphorylated form of src nor with unrelated phosphorylation sites.

Produktmerkmale: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Immunogen: Synthetic phosphopeptide conjuagted to KLHEpitope: phosphotyrosine 416AA Sequence: DNEpYTAR

Klon: 9A6

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Andere Bezeichnung: SRC1 (NCOA1 Produkte)

Synonyme: F-SRC-1 antikoerper, KAT13A antikoerper, RIP160 antikoerper, SRC1 antikoerper, bHLHe42 antikoerper, bHLHe74 antikoerper, si:dkey-5g7.6 antikoerper, si:dkey-34f16.6 antikoerper, NCOA1 antikoerper, SRC-1 antikoerper, nuclear receptor coactivator 1 antikoerper, NCOA1 antikoerper, ncoa1 antikoerper, Ncoa1 antikoerper

Hintergrund: Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an "open", active conformation which allows binding to other proteins via the SH2 and SH3 domain. Activated src phosphorylates a variety of substrates involved in the mitogenic signalling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication, and RNA processing.Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

Gen-ID: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha