Vimentin Antikörper
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- Target Alle Vimentin (VIM) Antikörper anzeigen
- Vimentin (VIM)
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Reaktivität
- Human, Maus, Hund
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Vimentin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Spezifität
- This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
- Aufreinigung
- Size exclusion chromatography
- Bestandteile
- incl. positive Control
- Immunogen
- Synthetic peptide conjugated to hemocyanin
- Klon
- 11H6
- Isotyp
- IgG
- Top Product
- Discover our top product VIM Primärantikörper
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- Applikationshinweise
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C/-80 °C
- Informationen zur Lagerung
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Haltbarkeit
- 12 months
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- Target
- Vimentin (VIM)
- Andere Bezeichnung
- Vimentin (VIM Produkte)
- Hintergrund
- Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
- Molekulargewicht
- 54 kDa
- Gen-ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Kaskade in der Apoptose
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