Caspase 9 Antikörper (Asp330, N-Term)

Produktnummer ABIN127028

Produktdetails anti-Caspase 9 Antikörper

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Bindungsspezifität: Asp330, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.

Aufreinigung: Affinity chromatography

Immunogen: Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human Caspase-9

Anwendungsinformationen

Applikationshinweise: Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry /Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS containing 50 % glycerol, 0.5 % BSA, and 0.02 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Details zu Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Andere Bezeichnung: Caspase-9 (CASP9 Produkte)

Hintergrund: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved Caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6

Gen-ID: 842

NCBI Accession: NP_001220

UniProt: P55211

Pathways: MAPK Signalweg, RTK Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Endopeptidase Activity