Insulin Antikörper

Produktnummer ABIN129507

Produktdetails anti-Insulin Antikörper

Target: Insulin (INS)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Insulin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Verwendungszweck: Insulin Antibody

Kreuzreaktivität (Details): This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur.

Produktmerkmale: Synonyms: mouse anti-insulin antibody, ILPR antibody, INS antibody, Insulin A chain antibody, Insulin B chain antibody, Insulin precursor antibody, IRDN antibody, Proinsulin antibody, Proinsulin precursor antibody

Aufreinigung: Protein A purified

Sterilität: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).

Immunogen Type: Native Protein

Klon: 2D11-H5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization.  Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.

Western Blot Dilution: 1:50 - 1:200

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Insulin

Target: Insulin (INS)

Andere Bezeichnung: INS (INS Produkte)

Hintergrund: Background: Recognizes the 51 amino acid (6 kDa) insulin polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids.  The main storage site for insulin is the pancreatic islets.  Antibodies to insulin are important as b-cell and tumor (insulinoma) markers.

Gen-ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation