Western Blotting (WB), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
Produktmerkmale
Concentration Definition: by UV absorbance at 280 nm
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein. Immunogentype:Recombinant
MAD2L1
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
This protein A purified antibody has been tested for use in immunoprecipitation and by western blot. This antibody is not applicable for immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14 Synonyms: Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody