S100 Protein (S100) Antikörper

Produktnummer ABIN129527

Produktdetails

Target: S100 Protein (S100)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
Immunogentype:Native

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This Protein A purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Antigendetails

Target: S100 Protein (S100)

Andere Bezeichnung: S100 Protein (S100 Produkte)

Synonyme: S100 antikoerper, S100-alpha antikoerper, S100A antikoerper, NEF antikoerper, S100-B antikoerper, S100beta antikoerper, S100A1 antikoerper, AI266795 antikoerper, S100a antikoerper, S100 calcium binding protein A1 antikoerper, S100 calcium binding protein B antikoerper, S100 calcium binding protein A13 antikoerper, S100A1 antikoerper, S100B antikoerper, S100a1 antikoerper, S100a13 antikoerper

Hintergrund: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - bb homodimer (S-100b) and ab heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke.  Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Synonyms: S100 calcium-binding protein A1 S-100 protein subunit alpha S-100 protein alpha chain

Gen-ID: 6271, 30582715

UniProt: P02639

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine