SARS-CoV-2 NSP5 (3CL-Pro) Antikörper

Produktnummer ABIN129545

Produktdetails anti-SARS-CoV-2 NSP5 (3CL-Pro) Antikörper

Target: SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))

Reaktivität: SARS Coronavirus (SARS-CoV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SARS-CoV-2 NSP5 (3CL-Pro) Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
Immunogentype:Recombinant

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein A purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu SARS-CoV-2 NSP5 (3CL-Pro)

Target: SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))

Andere Bezeichnung: SARS 3Cl Protease (3CL-PRO, M-Pro Produkte)

Synonyme: pp1ab antikoerper, ORF1a polyprotein;ORF1ab polyprotein antikoerper, ORF1ab antikoerper

Substanzklasse: Viral Protein

Hintergrund: Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.
Synonyms: 3CL PRO antibody, 3CLp antibody, nsp5 antibody

Gen-ID: 1489680, 29837498

UniProt: P0C6U8