GFP Antikörper

Produktnummer ABIN129570

Hersteller Produkt- Nr.: 600-401-215

Produktdetails anti-GFP Antikörper

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-215

Hersteller: Rockland

Verwendungszweck: GFP Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Produktmerkmale: Synonyms: rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Aufreinigung: Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 1:200 - 1:3,000

Application Note: Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:120,000

IF Microscopy Dilution: 1:500 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.02 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. GFP antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (ABIN129570)

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Jo, Fonseca, Bocco, Fernandes, McAninch, Bolin, Da Conceição, Werneck-de-Castro, Ignacio, Egri, Németh, Fekete, Bernardi, Leitch, Mannan, Curry, Butterfield, Bassett, Williams, Gereben, Ribeiro et al.: "Type 2 deiodinase polymorphism causes ER stress and hypothyroidism in the brain. ..." in: The Journal of clinical investigation, Vol. 129, Issue 1, pp. 230-245, (2019) (PubMed).

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Tsuchiya, Ogawa, Koujin, Mori, Osakada, Kobayashi, Hiraoka, Haraguchi: "p62/SQSTM1 promotes rapid ubiquitin conjugation to target proteins after endosome rupture during xenophagy." in: FEBS open bio, Vol. 8, Issue 3, pp. 470-480, (2018) (PubMed).

Schäfer, Algarrahi, Savarino, Yang, Seager, Franck, Costa, Liu, Logvinenko, Adam, Mauney: "Mode of Surgical Injury Influences the Source of Urothelial Progenitors during Bladder Defect Repair." in: Stem cell reports, Vol. 9, Issue 6, pp. 2005-2017, (2018) (PubMed).

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Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP (GFP Produkte)

Hintergrund: Background: Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP Antibody is ideal for Cell Biology, Neuroscience and Cancer research.

UniProt: P42212