NCS1 Antikörper

Produktnummer ABIN129571

Hersteller Produkt- Nr.: 600-401-216

Produktdetails anti-NCS1 Antikörper

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-216

Hersteller: Rockland

Verwendungszweck: Frequenin Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Produktmerkmale: Synonyms: rabbit anti-FREQUENIN antibody, NCS-1, FLUP, FREQ, Neuronal calcium sensor 1, frequenin homolog antibody, Frequenin-like protein antibody, NCS 1 antibody, NCS1 antibody

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:100-1:2500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Andere Bezeichnung: Ncs1 (NCS1 Produkte)

Hintergrund: Background: Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.

Gen-ID: 14299

NCBI Accession: NP_062655

UniProt: Q8BNY6