LIM Domain Binding 2 Protein Antikörper (AA 100-125)

Produktnummer ABIN129586

Hersteller Produkt- Nr.: 600-401-459

Produktdetails anti-LIM Domain Binding 2 Protein Antikörper

Target: LIM Domain Binding 2 Protein (LDB2) (LIM Domain Binding 2 (LDB2))

Bindungsspezifität: AA 100-125

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Binding 2 Protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-459

Hersteller: Rockland

Verwendungszweck: Ldb2 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse LDB2.

Produktmerkmale: Synonyms: rabbit anti-LDB2 antibody, rabbit anti-LDB2/CLIM1 antibody, rabbit anti-CLIM1 antibody, LDB-2, CLIM-1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein 1, LIM domain-binding factor CLIM1, Nuclear LIM interactor

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 100-125 of Mouse LDB2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu LIM Domain Binding 2 Protein

Target: LIM Domain Binding 2 Protein (LDB2) (LIM Domain Binding 2 (LDB2))

Andere Bezeichnung: Ldb2 (LDB2 Produkte)

Hintergrund: Background: LDB2 (LIM homeobox protein cofactor CLIM-1a).  It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1.  LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95 % , 73 % and 62 % amino acid identity with mouse Ldb1, respectively.

Gen-ID: 16826, 116642898

UniProt: O55203

Pathways: Stem Cell Maintenance