USP18 Antikörper (AA 25-50)

Produktnummer ABIN129593

Hersteller Produkt- Nr.: 600-401-476

Produktdetails anti-USP18 Antikörper

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Bindungsspezifität: AA 25-50

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser USP18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-476

Hersteller: Rockland

Verwendungszweck: UBP43 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against human UBP43 protein.

Produktmerkmale: Synonyms: rabbit anti-UBP43 antibody, rabbit anti-USP18 antibody, rabbit anti-ISG43 antibody, 43 kDa ISG15 specific protease antibody, hUBP43 antibody, Interferon Stimulated Gene 43 kD antibody, ISG15 Specific Processing Protease antibody, ISG43 antibody, Ubiquitin Specific Peptidase 18 antibody, Ubl carboxyl-terminal hydrolase 18, Ubl thioesterase 18

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to UBP43 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.9 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu USP18

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Andere Bezeichnung: USP18 (USP18 Produkte)

Hintergrund: Background: UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported.  Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Gen-ID: 11274, 32313610

UniProt: Q9UMW8