RAD52 Antikörper (AA 36-0)

Produktnummer ABIN129613

Produktdetails anti-RAD52 Antikörper

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Bindungsspezifität: AA 36-0

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD52 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360 - 375 of the Human Rad 52 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in western blotting using HeLa nuclear extracts.  The antibody is also functional by ELISA.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Andere Bezeichnung: RAD52 (RAD52 Produkte)

Synonyme: Rad52yh antikoerper, RAD52 antikoerper, zgc:112400 antikoerper, LOC100223085 antikoerper, MGC115453 antikoerper, RAD52 homolog, DNA repair protein antikoerper, RAD52 homolog (S. cerevisiae) antikoerper, RAD52 homolog, DNA repair protein S homeolog antikoerper, recombinase antikoerper, DNA repair and recombination protein, putative antikoerper, RAD52 protein antikoerper, RAD52 antikoerper, Rad52 antikoerper, rad52 antikoerper, rad52.S antikoerper

Hintergrund: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.   Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene.  The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).
Synonyms: DNA repair protein RAD52 homolog antibody, RAD 52 antibody, RAD52 homolog (S. cerevisiae) antibody, RAD52 homolog antibody, Recombination protein RAD52 antibody, Rhabdomyosarcoma antigen MU RMS 40.23 antibody

Gen-ID: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: DNA Reparatur