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ATAD5 Antikörper (AA 63-76)

Rockland 600-401-688 ATAD5 Reaktivität: Human ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN129617
Hersteller Produkt- Nr.: 600-401-688
  • Target Alle ATAD5 Antikörper anzeigen
    ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))
    Bindungsspezifität
    • 2
    • 2
    • 1
    AA 63-76
    Reaktivität
    • 11
    • 3
    • 1
    Human
    Wirt
    • 11
    • 2
    Kaninchen
    Klonalität
    • 13
    Polyklonal
    Konjugat
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ATAD5 Antikörper ist unkonjugiert
    Applikation
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Hersteller Produkt- Nr.
    600-401-688
    Hersteller
    Rockland
    Verwendungszweck
    ELG-1 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human Elg1 protein.
    Produktmerkmale
    Synonyms: rabbit anti-ELG-1 antibody, ELG1, ELG 1, FRAG1, ATP (GTP)-binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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  • Applikationshinweise

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:3,000 - 1:15,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.50 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    ATAD5 (ATPase Family, AAA Domain Containing 5 (ATAD5))
    Andere Bezeichnung
    ATAD5 (ATAD5 Produkte)
    Hintergrund
    Background: ELG1 (also known as ATP(GTP)-binding protein or Chromosome fragility associated gene 1) is involved in a novel RFC complex that is probably involved in DNA damage and repair by ensuring replication fidelity. This antibody detects a band at about 120 kDa in Hela, A431, Jurkat and HEK193 cells. This corresponds to the band size seen in Kanellis P et al. It remains unclear why the band size detected is much less than the 207 kDa predicted in the protein sequence corresponding to CACC44537.2 (Q96QE3), but as our results correspond to those seen in Kanellis P et al. it is likely that the 120 kDa target is ELG1.
    Gen-ID
    79915, 21615526
    UniProt
    Q96QE3
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