GLI3 Antikörper (AA 30-60)

Produktnummer ABIN129623

Hersteller Produkt- Nr.: 600-401-694

Produktdetails anti-GLI3 Antikörper

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Bindungsspezifität: AA 30-60

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Hersteller Produkt- Nr.: 600-401-694

Hersteller: Rockland

Verwendungszweck: GLI3 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against human Gli-3 protein.

Produktmerkmale: Synonyms: Rabbit anti-GLI-3 antibody, Transcriptional activator GLI3, Gli 3, GLI3 form of 190 kDa, GLI3 form of 83 kDa

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 0.5 mg/mL - 5 μg/mL

Application Note: This antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:6,000 - 1:30,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GLI3 Antikörper (ABIN129623)

Hehemann, Kalmanek, Choe, Dynda, Hu, Quek, Harrington, Stupp, McVary, Podlasek: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: GLI3 (GLI3 Produkte)

Hintergrund: Background: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.

Gen-ID: 2737, 119393899

UniProt: P10071

Pathways: Hedgehog Signalweg