GLI3 Antikörper (AA 41-57)

Produktnummer ABIN129623

Produktdetails anti-GLI3 Antikörper

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Bindungsspezifität: AA 41-57

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität: Schimpanse, Squirrel Monkey (Saimiri spec.), Xenopus laevis, Huhn, Hund, Wachtel

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to amino acids 41-57 of human Gli-3 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-GLI3 Antikörper (ABIN129623)

Hehemann, Kalmanek, Choe, Dynda, Hu, Quek, Harrington, Stupp, McVary, Podlasek: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: Gli-3 (GLI3 Produkte)

Synonyme: ACLS antikoerper, GCPS antikoerper, GLI3-190 antikoerper, GLI3FL antikoerper, PAP-A antikoerper, PAPA antikoerper, PAPA1 antikoerper, PAPB antikoerper, PHS antikoerper, PPDIV antikoerper, xgli3 antikoerper, AI854843 antikoerper, AU023367 antikoerper, Bph antikoerper, Pdn antikoerper, Xt antikoerper, add antikoerper, im:7142603 antikoerper, GLI3 antikoerper, GLI family zinc finger 3 antikoerper, GLI family zinc finger 3 L homeolog antikoerper, GLI-Kruppel family member GLI3 antikoerper, GLI3 antikoerper, Gli3 antikoerper, gli3.L antikoerper, gli3 antikoerper

Hintergrund: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development.  Two isoforms of human Gli-3 have been reported.  One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.
Synonyms: Gli-3, Zinc Finger Protein Gli-3, GLI-Kruppel family member GLI-3

Gen-ID: 2737, 119393899

UniProt: P10071

Pathways: Hedgehog Signalweg