GLI2 Antikörper (Internal Region)

Produktnummer ABIN129624

Hersteller Produkt- Nr.: 600-401-695

Produktdetails anti-GLI2 Antikörper

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Bindungsspezifität: Internal Region

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-695

Hersteller: Rockland

Verwendungszweck: Gli2 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against mouse Gli-2 protein.

Produktmerkmale: Synonyms: rabbit anti-Gli-2 antibody, Gli 2, Gli2, zinc finger protein GLI2, Tax helper protein antibody, Thp antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 2 μg/mL to 20 μg/mL

Application Note: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by WB and IHC.  Multiple splice variants have been reported for this protein.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:15,000 - 1:60,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.02 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-GLI2 Antikörper (ABIN129624)

Guntur, Reinhold, Cuellar, Naski: "Conditional ablation of Pten in osteoprogenitors stimulates FGF signaling." in: Development (Cambridge, England), Vol. 138, Issue 7, pp. 1433-44, (2011) (PubMed).

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: Gli2 (GLI2 Produkte)

Hintergrund: Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, and postaxial polydactyly types A1 and B. Anti-Gli 2 Antibody is useful for researchers interested in transcription factor activities, DNA binding, and chromatin binding research.

Gen-ID: 14633, 124487481

UniProt: Q8K0K3

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis