TUBA1B Antikörper (AA 425-451)

Produktnummer ABIN129653

Produktdetails anti-TUBA1B Antikörper

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Bindungsspezifität: AA 425-451

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TUBA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Verwendungszweck: Alpha-Tubulin Antibody

Kreuzreaktivität (Details): Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.

Produktmerkmale: Synonyms: rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Aufreinigung: The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:90,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Anti-Tubulin Loading Control Antibody at -20° C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TUBA1B Antikörper (ABIN129653)

Garland, Comet, Wu, Radzisheuskaya, Rib, Vitting-Seerup, Lloret-Llinares, Sandelin, Helin, Jensen: "A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2." in: Cell reports, Vol. 29, Issue 7, pp. 1800-1811.e6, (2019) (PubMed).

Kim, Kim: "Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3." in: Scientific reports, Vol. 8, Issue 1, pp. 8680, (2018) (PubMed).

Brown, Dayan, Wong, Kaczmarek, Hope, Pederson, Arnet, Goodall, Russell, Sadlon, Barry: "FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1." in: Oncotarget, Vol. 9, Issue 45, pp. 27708-27727, (2018) (PubMed).

Lloret-Llinares, Karadoulama, Chen, Wojenski, Villafano, Bornholdt, Andersson, Core, Sandelin, Jensen: "The RNA exosome contributes to gene expression regulation during stem cell differentiation." in: Nucleic acids research, Vol. 46, Issue 21, pp. 11502-11513, (2018) (PubMed).

Ghosh, Abdelwahab, Reeber, Reidel, Marklew, Garrison, Lee, Dang, Herring, Glish, Kesimer, Tarran: "Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression." in: Scientific reports, Vol. 7, pp. 46239, (2018) (PubMed).

Kapadia, Bijelić, Zhao, Ma, Stojanovich, Milošević, Andjus, Šakić: "Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling." in: Acta neuropathologica communications, Vol. 5, Issue 1, pp. 70, (2018) (PubMed).

Liao, Ren, Peterson, Marshak, Yau, Gamlin, Dacey: "Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations." in: The Journal of comparative neurology, Vol. 524, Issue 14, pp. 2845-72, (2017) (PubMed).

Dilworth, Upadhyay, Bonnafous, Edoo, Bourbigot, Pesek-Jardim, Gudavicius, Serpa, Petrotchenko, Borchers, Nelson, Mackereth: "The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module." in: Nucleic acids research, Vol. 45, Issue 20, pp. 11989-12004, (2017) (PubMed).

Lloret-Llinares, Mapendano, Martlev, Lykke-Andersen, Jensen: "Relationships between PROMPT and gene expression." in: RNA biology, Vol. 13, Issue 1, pp. 6-14, (2016) (PubMed).

Li, Dou, Li, Zhang, Zeng, Song: "Nicotinamide ameliorates palmitate-induced ER stress in hepatocytes via cAMP/PKA/CREB pathway-dependent Sirt1 upregulation." in: Biochimica et biophysica acta, Vol. 1853, Issue 11 Pt A, pp. 2929-36, (2015) (PubMed).

Besche, Sha, Kukushkin, Peth, Hock, Kim, Gygi, Gutierrez, Liao, Dick, Goldberg: "Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates." in: The EMBO journal, Vol. 33, Issue 10, pp. 1159-76, (2014) (PubMed).

Gudavicius, Dilworth, Serpa, Sessler, Petrotchenko, Borchers, Nelson: "The prolyl isomerase, FKBP25, interacts with RNA-engaged nucleolin and the pre-60S ribosomal subunit." in: RNA (New York, N.Y.), Vol. 20, Issue 7, pp. 1014-22, (2014) (PubMed).

Jakobsen, Sørensen, Brøndum, Daugaard, Thomsen, Nielsen: "Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells." in: Journal of molecular signaling, Vol. 8, Issue 1, pp. 9, (2013) (PubMed).

Balamurugan, Sharan, Klarmann, Zhang, Coppola, Summers, Roger, Morrison, Keller, Sterneck: "FBXW7α attenuates inflammatory signalling by downregulating C/EBPδ and its target gene Tlr4." in: Nature communications, Vol. 4, pp. 1662, (2013) (PubMed).

Hawkins, Oliaro, Kallies, Belz, Filby, Hogan, Haynes, Ramsbottom, Van Ham, Kinwell, Seddon, Davies, Tarlinton, Lew, Humbert, Russell: "Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity." in: Nature communications, Vol. 4, pp. 1801, (2013) (PubMed).

Grewe, Hoffmann, Ohs, Blissenbach, Brandt, Tippler, Grunwald, Uberla: "Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag." in: Journal of virology, Vol. 86, Issue 6, pp. 2990-3002, (2012) (PubMed).

Sarkar, Sharan, Wang, Pawar, Cantwell, Johnson, Morrison, Wang, Sterneck: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).

Hall, Cowan: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).

Details zu TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Andere Bezeichnung: TUBA1B (TUBA1B Produkte)

Hintergrund: Background: Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.

Gen-ID: 17986283

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase