beta Actin Antikörper (AA 359-368)

Produktnummer ABIN129657

Produktdetails anti-beta Actin Antikörper

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Bindungsspezifität: AA 359-368

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser beta Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität: Ratte (Rattus), Human, Affe, Schaf, Rind (Kuh), Ziege, Meerschweinchen, Schwein, Hamster

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

Isotyp: IgG

Produktspezifische Information:

Für welche Zwecke kann der beta-Actin-Antikörper ABIN129657 verwendet werden? Dieser polyklonale, unkonjugierte Kaninchen-beta-Actin-Antikörper eignet sich für den Nachweis von beta-Actin (ACTB) durch Immunhistochemie, ELISA und Western Blotting. Der Antikörper erkennt humanes beta-Actin.

Welche Validierungsdaten liegen für diesen beta-Actin-Antikörper vor? Der Antikörper wurde bisher in 31 Publikationen verwendet, die unten aufgeführt sind und bei Pubmed eingesehen werden können. Es sind 10 Produktbilder verfügbar, die die Leistung des beta-Actin-Antikörpers in Western Blots zeigen. Nutzen Sie unseren hochwertigen Antikörper, um beta-Actin zuverlässig nachzuweisen.

Was ist die Funktion von Actin? Actin ist ein hochkonserviertes Protein, das zu Filamenten polymerisiert, die vernetzte Netzwerke im Zytoplasma von Zellen bilden. Actin kommt sowohl in monomerer (G-Actin) als auch in polymerer Form (F-Actin) vor, die beide wichtige Funktionen wie Zellmotilität und Kontraktion erfüllen. Neben ihrer Rolle im zytoplasmatischen Zytoskelett sind G- und F-Aktin auch im Zellkern lokalisiert und regulieren die Gentranskription, die Motilität und die Reparatur von beschädigter DNA. (UniProt)

Anwendungsinformationen

Applikationshinweise: Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-beta Actin Antikörper (ABIN129657)

Olive, Qin, DeCristo, Laszewski, Greathouse, McAllister: "Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies." in: PLoS ONE, Vol. 13, Issue 6, pp. e0198790, (2019) (PubMed).

Dai, Luftig: "Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 5, pp. 1727-1736, (2019) (PubMed).

Borroni, Emanuelli, Shah, Ilić, Apel-Sarid, Paolini, Manikoth Ayyathan, Koganti, Levy-Cohen, Blank: "Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin." in: Aging cell, Vol. 17, Issue 2, (2018) (PubMed).

Coleman, Ha, Haeryfar, Barr, Kim: "Cathepsin B plays a key role in optimal production of the influenza A virus." in: Journal of virology & antiviral research, Vol. 2018, pp. 1-20, (2018) (PubMed).

Lodola, Laforenza, Cattaneo, Ruffinatti, Poletto, Massa, Tancredi, Zuccolo, Khdar, Riccardi, Biggiogera, Rosti, Guerra, Moccia: "VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells." in: Oncotarget, Vol. 8, Issue 56, pp. 95223-95246, (2017) (PubMed).

Chen, Wang, Man, Li, Shan: "Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice." in: Oncology letters, Vol. 13, Issue 4, pp. 2207-2215, (2017) (PubMed).

Rae, Hocum, Bii, Deeg, Trobridge: "A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis." in: Oncotarget, Vol. 6, Issue 31, pp. 30664-74, (2016) (PubMed).

Koh, Powell, Blake, Hoffman, Hopkins, Feng: "Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel." in: Oncology reports, Vol. 34, Issue 3, pp. 1589-98, (2016) (PubMed).

Campos, Qiu, Gomero, Wakefield, Horner, Brutkowski, Han, Solecki, Frase, Bongiovanni, dAzzo: "Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier." in: Nature communications, Vol. 7, pp. 11876, (2016) (PubMed).

Hopkins, Feng, Liu, Parker, Koh: "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." in: International journal of oncology, Vol. 46, Issue 5, pp. 2267-76, (2015) (PubMed).

Maragkoudaki, Vaiopoulou, Theodoropoulos, Legaki, Sechi, Karamanolis, Zografos, Gazouli: "Specific detection of OCT4 isoforms in inflammatory bowel disease." in: Gut pathogens, Vol. 7, pp. 25, (2015) (PubMed).

Zha, Xia, Ding, Choi, Yang, Dong, Yan, Huang, Ding: "MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression." in: Cell death & disease, Vol. 5, pp. e1417, (2015) (PubMed).

Nelson, Estus, Abner, Parikh, Malik, Neltner, Ighodaro, Wang, Wilfred, Wang, Kukull, Nandakumar, Farman, Poon, Corrada, Kawas, Cribbs, Bennett, Schneider, Larson, Crane, Valladares, Schmitt, Kryscio et al.: "ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. ..." in: Acta neuropathologica, Vol. 127, Issue 6, pp. 825-43, (2015) (PubMed).

Wang, Choi, Ding, Yang, Ngoka, Lee, Zha, Mao, Jin, Ren, Cowell, Huang, Shi, Cui, Ding: "HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation." in: BMC genomics, Vol. 14, pp. 830, (2014) (PubMed).

Dragoni, Laforenza, Bonetti, Reforgiato, Poletto, Lodola, Bottino, Guido, Rappa, Pareek, Tomasello, Guarrera, Cinelli, Aronica, Guerra, Barosi, Tanzi, Rosti, Moccia: "Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis." in: PLoS ONE, Vol. 9, Issue 3, pp. e91099, (2014) (PubMed).

Dragoni, Turin, Laforenza, Potenza, Bottino, Glasnov, Prestia, Ferulli, Saitta, Mosca, Guerra, Rosti, Luinetti, Ganini, Porta, Pedrazzoli, Tanzi, Montagna, Moccia: "Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma." in: BioMed research international, Vol. 2014, pp. 739494, (2014) (PubMed).

Wang, Danaher, Miller, Berger, Nubia, Wilfred, Neltner, Norris, Nelson: "Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells." in: Genomics, proteomics & bioinformatics, Vol. 12, Issue 1, pp. 19-30, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." in: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Laforenza, Scaffino, Gastaldi: "Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes." in: PLoS ONE, Vol. 8, Issue 1, pp. e54474, (2013) (PubMed).

Lodola, Laforenza, Bonetti, Lim, Dragoni, Bottino, Ong, Guerra, Ganini, Massa, Manzoni, Ambudkar, Genazzani, Rosti, Pedrazzoli, Tanzi, Moccia, Porta: "Store-operated Ca2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients." in: PLoS ONE, Vol. 7, Issue 9, pp. e42541, (2013) (PubMed).

Details zu beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: beta Actin (ACTB Produkte)

Synonyme: BRWS1 antikoerper, PS1TP5BP1 antikoerper, ACTA1 antikoerper, Actx antikoerper, E430023M04Rik antikoerper, beta-actin antikoerper, ps1tp5bp1 antikoerper, act-2 antikoerper, actb antikoerper, B-actin antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, A antikoerper, ACT5C antikoerper, Ac5C antikoerper, Act antikoerper, Act-5C antikoerper, Act5 antikoerper, Act5c antikoerper, Actin antikoerper, Actin/BAP47 antikoerper, Actin5C antikoerper, BAP47 antikoerper, Bap47 antikoerper, CG4027 antikoerper, Dmel\\CG4027 antikoerper, M32055 antikoerper, T11 antikoerper, act antikoerper, act 5C antikoerper, act5C antikoerper, actin antikoerper, actin5C antikoerper, anon-EST:fe2D2 antikoerper, beta-actin/Bap47 antikoerper, cyt5C antikoerper, l(1)G0009 antikoerper, l(1)G0010 antikoerper, l(1)G0025 antikoerper, l(1)G0079 antikoerper, l(1)G0117 antikoerper, l(1)G0177 antikoerper, l(1)G0245 antikoerper, l(1)G0330 antikoerper, l(1)G0420 antikoerper, l(1)G0486 antikoerper, Bact antikoerper, ACT-5 antikoerper, ACTB antikoerper, B-ACTZF antikoerper, actba antikoerper, bact antikoerper, bactin1 antikoerper, bactzf antikoerper, wu:fd18f05 antikoerper, A26C1B antikoerper, POTE2alpha antikoerper, POTEACTIN antikoerper, Beta-actin antikoerper, actin beta antikoerper, actin, beta antikoerper, cytoplasmic actin OlCA1 antikoerper, beta-actin antikoerper, Actin 5C antikoerper, actin gamma 1 antikoerper, actin, beta 1 antikoerper, actin, beta L homeolog antikoerper, beta actin antikoerper, POTE ankyrin domain family member F antikoerper, actin, beta like 2 antikoerper, actin, cytoplasmic 1 antikoerper, ACTB antikoerper, Actb antikoerper, actb antikoerper, LOC100174883 antikoerper, LOC100304816 antikoerper, Act5C antikoerper, ACTG1 antikoerper, actb1 antikoerper, actb.L antikoerper, LOC100136352 antikoerper, POTEF antikoerper, ACTBL2 antikoerper, LOC109073280 antikoerper

Hintergrund: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Gen-ID: 4501885

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction