CBLC Antikörper (C-Term)

Produktnummer ABIN129659

Hersteller Produkt- Nr.: 600-401-889

Produktdetails anti-CBLC Antikörper

Target: CBLC (Cbl proto-oncogene C (CBLC))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CBLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 600-401-889

Hersteller: Rockland

Verwendungszweck: Cbl-c Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Cbl-c protein.

Produktmerkmale: Synonyms: rabbit anti-Cbl-c Antibody, E3 ubiquitin-protein ligase CBL-C, RING finger protein 57, RING-type E3 ubiquitin transferase CBL-C, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C, CBLC, CBL3, RNF57

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal portion of Human Cbl-c.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, immunoprecipitation and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~52 kDa in size corresponding to Cbl-c by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CBLC

Target: CBLC (Cbl proto-oncogene C (CBLC))

Andere Bezeichnung: CBLC (CBLC Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57.  Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation.  Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed).  Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation.  Cbl-c knock out mice show no obvious phenotype.  Thus, the physiological role of Cbl-c is not known.

Gen-ID: 23624, 125987803

UniProt: Q9ULV8

Pathways: EGFR Signaling Pathway