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HMGN1 Antikörper (pSer20, pSer24)

Rockland 600-401-898 HMGN1 Reaktivität: Human WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN129664
Hersteller Produkt- Nr.: 600-401-898
  • Target Alle HMGN1 Antikörper anzeigen
    HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
    Bindungsspezifität
    • 17
    • 15
    • 15
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer20, pSer24
    Reaktivität
    • 63
    • 19
    • 15
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 64
    • 2
    Kaninchen
    Klonalität
    • 64
    • 2
    Polyklonal
    Konjugat
    • 32
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser HMGN1 Antikörper ist unkonjugiert
    Applikation
    • 41
    • 28
    • 26
    • 24
    • 23
    • 14
    • 10
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Hersteller Produkt- Nr.
    600-401-898
    Hersteller
    Rockland
    Verwendungszweck
    HMGN phospho S20/S24 Antibody
    Kreuzreaktivität (Details)
    This affinity-purified antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions).
    Produktmerkmale
    Synonyms: rabbit anti-HMGN pS20/pS24 antibody, Non-histone chromosomal protein HMG-14, High mobility group nucleosome binding domain containing protein 1 antibody, High mobility group protein 14 antibody, High-mobility group nucleosome binding domain 1 antibody, HMG14 antibody, HMGN-1, HMGN 1, HMG 14
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 19-28 of human HMGN protein (see below).

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
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  • Applikationshinweise

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-HMGN pS20-pS24 affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17 kDa in size corresponding to phosphorylated HMGN proteins by western blotting in the appropriate cell lysate or extract.  Less than 0.5 % reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS20 and pS24 of HMGN proteins.  The antibody was tested against HMGN1 by performing a standard phosphorylation in vitro assay using HMGN1 as a substrate and Msk1 as a kinase (known as a main effector kinase of MAPK signaling pathway and in vivo kinase for HMGN1). This kinase specifically phosphorylates S20 and S24 of HMGN1 (S24 and S28 of HMGN2).  The reaction was resolved on SDS PAGE and immunoblot, using antibody diluted at 1:1,000 and 1:5,000. The antibody shows strong positive signal for Msk-phosphorylated HMGN1 and no cross-reaction for non-treated HMGN1.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.20 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
    Andere Bezeichnung
    HMGN1 (HMGN1 Produkte)
    Hintergrund
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis.  The sequence immunized is conserved in several species.  As such, this reagent is designed as a ''universal'' reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels.  HMGs were arbitrarily classed as a specific type of non-histone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family.  Each HMG family has a characteristic functional sequence motif.  The functional motif of the HMGB family is called the ''HMG-box,'' that of the HMGN family, the ''nucleosomal binding domain,'' and that of the HMGA family, the ''AT-hook.'' The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as ''HMG motif proteins.'' Anti-HMGN pS20-pS24 Antibody is ideal for researchers interested in chromatin binding and Nuclear Signaling research.
    Gen-ID
    3091, 386779
    UniProt
    P05114
    Pathways
    Chromatin Binding
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