CTCFL Antikörper (AA 1-30)

Produktnummer ABIN129668

Hersteller Produkt- Nr.: 600-401-907

Produktdetails anti-CTCFL Antikörper

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Bindungsspezifität: AA 1-30

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTCFL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 600-401-907

Hersteller: Rockland

Verwendungszweck: BORIS Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against the human BORIS protein.

Produktmerkmale: Synonyms: rabbit anti-BORIS Antibody, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites antibody, CCCTC binding factor (zinc finger protein) like antibody, CCCTC-binding factor antibody, CTCF paralog antibody, CTCF-like, cancer/testis antigen 27, CT27, CTCF-T

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: BORIS affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.28 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store BORIS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CTCFL Antikörper (ABIN129668)

Janssen, Moscona, Elchebly, Papadakis, Redpath, Wang, Rubin, van Kempen, Spatz: "BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells." in: Cell death discovery, Vol. 6, pp. 1, (2020) (PubMed).

Rosa-Garrido, Ceballos, Alonso-Lecue, Abraira, Delgado, Gandarillas: "A cell cycle role for the epigenetic factor CTCF-L/BORIS." in: PLoS ONE, Vol. 7, Issue 6, pp. e39371, (2012) (PubMed).

Macaluso, Caracciolo, Rizzo, Sun, Montanari, Russo, Bellipanni, Khalili, Giordano: "Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 9, pp. 1841-50, (2012) (PubMed).

Fiorentino, Macaluso, Miranda, Montanari, Russo, Bagella, Giordano: "CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancer." in: Molecular cancer research : MCR, Vol. 9, Issue 2, pp. 225-33, (2011) (PubMed).

Hines, Bazarov, Mukhopadhyay, Yaswen: "BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas." in: PLoS ONE, Vol. 5, Issue 3, pp. e9738, (2010) (PubMed).

Dougherty, Ichim, Liu, Reznik, Min, Ghochikyan, Agadjanyan, Reznik: "Selective apoptosis of breast cancer cells by siRNA targeting of BORIS." in: Biochemical and biophysical research communications, Vol. 370, Issue 1, pp. 109-12, (2008) (PubMed).

Details zu CTCFL

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Andere Bezeichnung: CTCFL (CTCFL Produkte)

Hintergrund: Background: Anti-BORIS antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as  CCCTC-binding factor-like protein, is  normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.  However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.  Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.  BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.  BORIS is found in both the nucleus and cytoplasm. BORIS antibody can be used to investigate epigenetic regulation.

Gen-ID: 140690, 20805280

UniProt: Q8NI51