CTCFL Antikörper (AA 9-26)

Produktnummer ABIN129668

Produktdetails anti-CTCFL Antikörper

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Bindungsspezifität: AA 9-26

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTCFL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität: Schimpanse

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 9-26 of human BORIS protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.28 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-CTCFL Antikörper (ABIN129668)

Janssen, Moscona, Elchebly, Papadakis, Redpath, Wang, Rubin, van Kempen, Spatz: "BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells." in: Cell death discovery, Vol. 6, pp. 1, (2020) (PubMed).

Rosa-Garrido, Ceballos, Alonso-Lecue, Abraira, Delgado, Gandarillas: "A cell cycle role for the epigenetic factor CTCF-L/BORIS." in: PLoS ONE, Vol. 7, Issue 6, pp. e39371, (2012) (PubMed).

Macaluso, Caracciolo, Rizzo, Sun, Montanari, Russo, Bellipanni, Khalili, Giordano: "Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 9, pp. 1841-50, (2012) (PubMed).

Fiorentino, Macaluso, Miranda, Montanari, Russo, Bagella, Giordano: "CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancer." in: Molecular cancer research : MCR, Vol. 9, Issue 2, pp. 225-33, (2011) (PubMed).

Hines, Bazarov, Mukhopadhyay, Yaswen: "BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas." in: PLoS ONE, Vol. 5, Issue 3, pp. e9738, (2010) (PubMed).

Dougherty, Ichim, Liu, Reznik, Min, Ghochikyan, Agadjanyan, Reznik: "Selective apoptosis of breast cancer cells by siRNA targeting of BORIS." in: Biochemical and biophysical research communications, Vol. 370, Issue 1, pp. 109-12, (2008) (PubMed).

Details zu CTCFL

Target: CTCFL (CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL))

Andere Bezeichnung: BORIS (CTCFL Produkte)

Synonyme: BORIS antikoerper, CT27 antikoerper, CTCF-T antikoerper, HMGB1L1 antikoerper, dJ579F20.2 antikoerper, CTCFL antikoerper, Boris antikoerper, OTTMUSG00000016680 antikoerper, CCCTC-binding factor like antikoerper, CCCTC-binding factor (zinc finger protein)-like antikoerper, CTCFL antikoerper, Ctcfl antikoerper

Hintergrund: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as  CCCTC-binding factor-like protein, is  normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.  However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.  Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.  BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.  BORIS is found in both the nucleus and cytoplasm.
Synonyms: Brother of the regulator of imprinted sites antibody, CCCTC binding factor (zinc finger protein) like antibody, CCCTC-binding factor antibody, CTCF paralog antibody, CTCF T antibody

Gen-ID: 140690, 20805280

UniProt: Q8NI51