Histone H2A Variant (HIS2AV) (Internal Region), (pSer137) Antikörper

Produktnummer ABIN129671

+[w1118] controls) or (D) a GeneSwitch inducible driver (Act88FGS, +RU486 compared with -RU486 [vehicle alone] sibling controls). (E) Quantification of mitoses per whole dissected midgut (assayed by anti- pH 3 immunostaining) at indicted ages, genotypes described above, bars represent mean ± SE, n = 25-30. (F) Immunostaining of dissected intestines to assess epithelial integrity of posterior midguts at indicated ages, genotypes described above, pH 3 (green), armadillo (""Arm"", membrane, red), and DAPI (blue). Representative images shown. (G-H) Lineage tracing from ISCs using FRT recombination of a split alpha-tubulin-lacZ transgene (in Act88FG4>+[w1118, controls] or Act88FG4>UAS-Mei-9RNAi genetic background). (G) Changes in clone size (cell per clone form posterior midgut) at indicated ages, represented as box plot (median, red line), n = 25. (H) Representative images of lacZ clones from various genotypes at indicated ages, immunostaining of dissected midguts (posterior), anti-lacZ (green), and DAPI (blue). (I) Venn diagrams showing overlap of up-regulated genes (from dissected midguts) between Act88FG4>UAS-Mei-9RNAi and controls Act88FGal4>+(w1118) during aging (transcriptomes at 30 d, compared to Act88FGal4>+[w1118] controls at day 10). The threshold for genes included in the analysis was (i) changes in RPKM values of at least 2-fold up-regulated in intestine compared to young controls and (ii) a minimum RPKM value of 2. (J) Fold change (in intestinal transcriptome RPKM values, Day 30 Act88FG4>UAS-Mei-9RNAi/ Day 10 Act88FGal4>+[w1118] control [black bars] or Day 30 control/Day 10 control [gray bars]) of selected innate immune genes. Underlying data can be found in S1 Data. See also S1 and S2 Figs and S1 and S2 Tables. FRT, flippase recombination target, ISC, intestinal stem cell, pH 3, phospho Histone H3, mu-specific, muscle-specific, RPKM, reads per kbp per million reads, RU486, mifepristone. - figure provided by CiteAb. Source: PMID30036358">

Produktdetails

Target: Histone H2A Variant (HIS2AV)

Bindungsspezifität: Internal Region, pSer137

Reaktivität: Drosophila melanogaster

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Spezifität: This affinity purified anti-Histone H2AvD pS137 Antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pS137 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Reactivity against homologues from other sources is not known.

Produktmerkmale: Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.

Sterilität: Sterile filtered

Immunogen: Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Histone H2 AvD pS137 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2 vD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2 vD protein.

Kommentare:

Gene Name: HIS2AV

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/ml

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 ° C prior to restoration. For extended storage aliquot contents and freeze at -24 ° C or below. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen

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Antigendetails

Target: Histone H2A Variant (HIS2AV)

Andere Bezeichnung: Histone H2AvD

Synonyme: 5499 antikoerper, CG5499 antikoerper, Dmel\\CG5499 antikoerper, H2A antikoerper, H2A.F/Z antikoerper, H2A.X antikoerper, H2A.Z antikoerper, H2AV antikoerper, H2AV_DROM antikoerper, H2AX antikoerper, H2AZ antikoerper, H2Av antikoerper, H2AvD antikoerper, H2a.V antikoerper, H2av antikoerper, H2avD antikoerper, HIS antikoerper, HIS2AV antikoerper, HIS2AVD antikoerper, His antikoerper, His2 antikoerper, His2AV antikoerper, His2AvD antikoerper, HisH2Av antikoerper, Hist antikoerper, Hist2av antikoerper, gamma-H2Av antikoerper, gamma-HIS2AV antikoerper, gamma-His2Av antikoerper, gammaH2Av antikoerper, h2AvD antikoerper, his antikoerper, l(3)05146 antikoerper, l(3)810 antikoerper, l(3)97Dd antikoerper, l(3)L1602 antikoerper, Histone H2A variant antikoerper, His2Av antikoerper

Hintergrund: Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.
Synonyms: H2AvD protein antibody

Gen-ID: 43229, 17738227

UniProt: P08985