MLF1IP Antikörper (pThr78)

Produktnummer ABIN129686

Hersteller Produkt- Nr.: 600-401-930

Produktdetails anti-MLF1IP Antikörper

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Bindungsspezifität: pThr78

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MLF1IP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Hersteller Produkt- Nr.: 600-401-930

Hersteller: Rockland

Verwendungszweck: MLF1 phospho T78 Antibody

Kreuzreaktivität (Details): This antibody is specific for human MLF1IP protein phosphorylated at Thr78.

Produktmerkmale: Synonyms: rabbit anti-MLF1 pT78 antibody, rabbit anti-MLF1 Interacting Protein pT78 antibody, Centromere protein U, CENP-U, Centromere protein of 50 kDa, CENP-50, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, ICEN24, PBIP1, KLIP1

Aufreinigung: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against the non-phospho peptide.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein.  The immunogen peptide is phosphorylated at Thr78.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 20 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, IF, and IHC.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 65 kDa in size corresponding to MLF1IP protein by western blotting in the appropriately stimulated tissue, cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:25,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-MLF1IP Antikörper (ABIN129686)

Kang, Park, Yu, Soung, Yun, Bang, Seong, Yu, Garfield, Veenstra, Lee: "Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation." in: Molecular cell, Vol. 24, Issue 3, pp. 409-22, (2006) (PubMed).

Details zu MLF1IP

Target: MLF1IP (MLF1 Interacting Protein (MLF1IP))

Andere Bezeichnung: CENPU (MLF1IP Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as Centromere Protein U) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. Anti-MLF1 pT78 Antibody is useful for researchers interested in Cell Cycle Proteins, Chromatin, and Ubiquitin Research.

Gen-ID: 79682, 38016935

UniProt: Q71F23